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Human CD137L Stable 293T Cell Line

Catalog Number CBCL-463
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human CD137L gene

Application

Drug screening and biological assays

Background

CD137L is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) Ligand superfamily. After binding to it receptor TNFRSF9 (CD137), CD137L is involved in several immunological process, such as inducing the proliferation of activated T cells, activation-induced cell death. This cytokine is also expressed in carcinoma cell lines, and is believed to be involved in T cell-tumor cell interaction.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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