Human CD154 (CD4OL) Stable CHOK1 Cell Line

Catalog Number

CBCL-575

Price

Online Inquiry

Unit Quantity

1x10⁶ cells / 1.0 ml

Introduction

CHO-K1 cell line stable expressing exogenous human CD154 gene

Application

Drug screening and biological assays

Background

CD154, also named as CD40L ligand, is protein of TNF superfamily, mainly expressed on activated T cell, functioned as CD40 ligand, plays critical in immune response, such as B cell maturation, macrophage activation.

Product Information

Species	Mouse
Host Cell Line	CHOK1
Cell Type	Chinese hamster ovary cell line
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	F12K + 10% FBS + 5 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 30 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.