| Catalog Number | CBCL-377 |
| Price | Online Inquiry |
| Unit Quantity | 1x106 cells / 1.0 ml |
A549 cell line stable expressing exogenous CD228 gene
Drug screening and biological assays
CD228, also named as MFI2, is a member of the transferrin superfamily that binds to a single ferric iron with high affinity. CD228 expression is low in normal tissues but high in tumor and embryonic tissue.
| Category | Immune Molecules Cell Lines |
| Cell Line Name | Human CD228 Stable A549 Cell Line |
| Pharmaceutical Target | Immune Molecules |
| Species | Human |
| Host Cell Line | A549 |
| Cell Type | Human lung cancer cell line |
| Morphology | Epithelial |
| Growth Properties | Adherent |
| Selection Marker | Puromycin and hygromycin B |
| Seeding Density | Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio | Recommended a ratio of 1:4-1:5. |
| Doubling Time | Approximately 20 hours |
| Recommended Medium | McCoy's 5a + 10% FBS + 1 μg/ml puromycin |
| Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Stability | Stable in culture over 10 passages |
| QC | WB |
| Shipping | Shipping in dry ice |
| Biosafety Level | BSL-1 |
| Caution | For Research Use Only |