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Human CD27 Stable HT1080 Cell Line

Catalog Number CBCL-437
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HT1080 cell line stable expressing exogenous human CD27 gene

Application

Drug screening and biological assays

Background

CD27, also named as TNFRSF7, is a transmembrane protein belonging to the TNF receptor superfamily, and no only functioned as a co-stimulatory immune checkpoint molecule in T cell activation, but also play a critical role in regulating B-cell activation and immunoglobulin synthesis after binding with its ligand.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name Human CD27 Stable HT1080 Cell Line
Pharmaceutical Target Immune Molecules
Species Human
Host Cell Line HT1080
Cell Type Human fibrosarcoma cells
Morphology Fibroblast
Growth Properties Adherent
Selection Marker Puromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Doubling Time Approximately 48 hours
Recommended Medium RPMI1640 + 10% FBS + 0.5 μg/ml Puromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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