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Human CD28 Stable CHOK1 Cell Line

Catalog Number CBCL-632
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

CHOK1 cell line stable expressing exogenous human CD28 gene

Application

Drug screening and biological assays

Background

CD28 is a membrane protein of B7 receptor family and mainly expressed onT cells. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins, and provide co-stimulatory signals for T cell activation and survival.

Product Information

Species Mouse
Host Cell Line CHOK1
Cell Type Chinese hamster ovary cell line
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium RPMI1640 + 10% FBS + 6 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:8.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 24 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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