Human CD30 Stable HT1080 Cell Line
| Catalog Number |
CBCL-446 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable HT-1080 cell line expressing exogenous human CD30 gene
Application
Drug screening and biological assays
Background
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and a tumor marker. CD30 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.
Product Information
| Species |
Human |
| Host Cell Line |
HT1080 |
| Cell Type |
Human fibrosarcoma cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
RPMI1640 + 10% FBS + 1 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Fibroblast |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 40 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
