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Human CD30L Stable 293T Cell Line

Catalog Number CBCL-450
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human CD30L gene

Application

Drug screening and biological assays

Background

CD30L, belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while toinduce cell death and reduce cell proliferation of other lymphoma cell lines.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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