Human CD40 Stable HT1080 Cell Line

Catalog Number	CBCL-436
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HT1080 cell line stable expressing exogenous human CD40 gene

Application

Drug screening and biological assays

Background

CD40, also named as TNFRSFS, is a transmembrane protein of the TNF receptor superfamily, expressed on B cells, DC cells, macrophage, monocytes and plates, CD40 functioned as a costimulatory molecule and mediated broad immune and inflammatory response.

Product Information

Species	Human
Host Cell Line	HT1080
Cell Type	Human fibrosarcoma cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	RPMI1640 + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Monolayer
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 48 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.