Human CD47 Stable CHOK1 Cell Line
| Catalog Number |
CBCL-530 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
The CHO-K1-CD47-Stable Cell Line consists of recombinant CHO-K1 cells that stably overexpress human CD47 on their surface. The surface expression of CD47 is confirmed by FACS analysis.
Application
Drug screening and biological assays
Background
CD47 is a “don’t eat me” signal expressed on normal cells and tumor cells. It binds to SIRPα on macrophages, inhibiting phagocytosis. Tumor cells overexpress CD47 to evade immune surveillance, making it a target for cancer immunotherapy (e.g., anti-CD47 antibodies).
Product Information
| Category |
Immune Checkpoint Cell Lines |
| Cell Line Name |
CHO-K1-CD47-Stable Cell Line |
| Pharmaceutical Target |
Immune Molecules |
| Protein Family |
Protein family of scavenger receptors |
| Gene |
CD47 |
| NCBI Gene ID |
961 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:8. |
| Doubling Time |
Approximately 24 hours |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin |
| Freeze Medium |
95% complete growth medium + 5% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| QC |
WB |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
