Human CD64 (Luc)-Jurkat Reporter Cell
| Catalog Number |
CBCL-0007 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
A Jurkat reporter cell line that expresses the NFAT response element driving luciferase - expressing systems and the full - length human CD64 (Uniprot: P12314-1) has been engineered. This cell line can be used to evaluate the ADCP activity of antibodies in the presence of corresponding target cells.
Application
Determination of ADCP activity induced by antibodies
Background
FCGR1A encodes a high-affinity receptor for the Fc region of IgG. It is expressed on monocytes, macrophages, and dendritic cells. This receptor has a high binding affinity for monomeric IgG, allowing it to efficiently capture and internalize immune complexes. It is involved in the initiation of immune responses, the activation of immune cells, and the regulation of inflammation. It also plays a role in antibody-mediated phagocytosis and the clearance of apoptotic cells.
Product Information
| Category |
Fc Receptor Cell Lines |
| Cell Line Name |
Human CD64 (Luc)-Jurkat Reporter Cell |
| Protein Family |
Fc gamma receptor |
| Gene |
CD64/FCGR1A |
| NCBI Gene ID |
2209 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
