Human CD73-M Stable MC38 Cell Line

Catalog Number	CBCL-343
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable MC38 clone expressing exogenous human CD73 gene

Application

Drug screening and biological assays

Background

CD73 is a glycosylphosphatidylinositol (GPI) anchored cell surface protein that is encoded by NTSE gene, which plays a crucial role in switch on adenosinergic signaling. CD73 is highly expressed in breast cancer, colorectal cancer, ovarian cancer, gastric cancer, and gallbladder cancer and associated. This gene can also be used asa key regulatory molecule in cancer development.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human CD73-M Stable MC38 Cell Line
Pharmaceutical Target	Immune Molecules
Species	Mouse
Host Cell Line	MC38
Cell Type	Mouse colon cancer cell line
Morphology	Monolayer
Growth Properties	Adherent
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:10.
Doubling Time	Approximately 20 hours
Recommended Medium	RPMI 1640 + 10% FBS+ 5 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.