Human CD80 Stable 293T Cell Line

Catalog Number	CBCL-459
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human CD80 gene

Application

Drug screening and biological assays

Background

CD80, also named as B7-1, is type I membrane protein and belongs to immunoglobulin superfamily. CD80 is found on the surface of various immune cells, such as DCs, B cells, monocytes, etc. CD80 often works closely to CD86 (B7-2) in tandem to prime T cells after binding with their receptor CTLA4 and CD28.

Product Information

Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Monolayer
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 30 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.