Human CD94-NKG2A Stable CT26.WT Cell Line
| Catalog Number |
CBCL-411 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
CT26.WT cell line stable expressing exogenous human CD94 and NKG2A gene
Application
Drug screening and biological assays
Background
CD94 & NKG2A, also known as KLRD1 & CD159A. CD94 is a type II integral membrane protein belonging to lectin superfamily, which is plays arole as a receptor for the recognition of MHC class I Human Leukocyte Antigen (HLA)-E molecules by NK cells and some cytotoxic T-cells. NKG2A contains C-type lectin domain and belongs to the killer cell lectin-like receptor (KLR) family. KLR family is agroup of transmembrane proteins preferentially expressed in natural killer (NK) cells. CD94 can form disulfide-bonded heterodimer with NKG2A on the surface of NK cells. The CD94/NKG2A complex interacts with HLA-E on target cells and inhibit the cytotoxic activity of NK cells to prevent cell Iysis.
Product Information
| Species |
Mouse |
| Host Cell Line |
CT26.WT |
| Cell Type |
Mouse colon adenocarcinoma cell line |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
RPMI1640 + 10% FBS + 10 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:10. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
|
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
