Human CLDN18.1-Flag Stable 293T Cell Line
| Catalog Number |
CBCL-558 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
HEK293T cell line stable expressing exogenous human CLND18 gene isoform 1
Application
Drug screening and biological assays
Background
CLDN18.1 is the isoform 1 of the claudin 18 gene, which belong to the lager claudin family and play the important role in cell tight junction in epithelial cells. The other isoform, CLND18.2, is found overexpressed on gastrointestinal adenocarcinoma and pancreatic tumors. The identification as a tumor target of CLDN18.2 has led to the repaid progress of antibody treatment of gastrointestinal adenocarcinoma and pancreatic tumors.
Product Information
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
DMEM + 10% FBS + 0.5 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
