Human FAP Stable HT1080 Cell Line

Catalog Number	CBCL-307
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable HT-1080 cell line expressing exogenous human FAP gene

Application

Drug screening and biological assays

Background

FAP, also known as FAP alpha, is a type II transmembrane glycoprotein that belongs to the serine protease family, which is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. FAP is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Product Information

Species	Human
Host Cell Line	HT-1080
Cell Type	Human fibrosarcoma cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	RPMI1640 + 10% FBS + 1 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 30 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.