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Human FAP Stable HT1080 Cell Line

Catalog Number CBCL-307
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable HT-1080 cell line expressing exogenous human FAP gene

Application

Drug screening and biological assays

Background

FAP, also known as FAP alpha, is a type II transmembrane glycoprotein that belongs to the serine protease family, which is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. FAP is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Product Information

Species Human
Host Cell Line HT-1080
Cell Type Human fibrosarcoma cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium RPMI1640 + 10% FBS + 1 μg/ml Puromycin
Selection Marker Puromycin
Morphology Epithelial
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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