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Human FGL1 Stable MC38 Cell Line

Catalog Number CBCL-420
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable MC38 cell line expressing exogenous human FGL1 gene

Application

Drug screening and biological assays

Background

FGL1 is also known as HFREP-1, is a member of the fibrinogen family of proteins. FGL-1is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of that are common to all members of the fibrinogen family. However, FGL-1 lacks the platelet-binding site, cross-linking region, and thrombin-sensitive site which allow the other members of the fibrinogen family to aid in fibrin clot formation. FGL-1 may play a role in the development of hepatocellular carcinomas.

Product Information

Species Mouse
Host Cell Line MC38 cell line
Cell Type Mouse colon cancer cell line
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium RPMI1640 + 10% FBS + 5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Epithelial
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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