Human FIBCD1-FGL1 Stable CHOK1 Cell Line
| Catalog Number |
CBCL-555 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
CHOK1 cell line stable expressing exogenous human FIBCD1-FGL1 fusion gene
Application
Drug screening and biological assays
Background
Fibrinogen-Like protein 1 (FGL1) is a liver specific secreted protein which belongs to the fibrinogen superfamily. FGL1 plays an important role in the development of hepatocellular carcinoma, with its elevated level in plasma of cancer patient indicating poor prognoses. Further studies showed that FGL1 can inhibit antigen-specific T cell activation through binding with immune checkpoint inhibiting molecule LAG3 through its fibrinogen-like domain independent of MHCII molecules.
Product Information
| Category |
Immune Molecules Cell Lines |
| Cell Line Name |
Human FIBCD1-FGL1 Stable CHOK1 Cell Line |
| Pharmaceutical Target |
Immune Molecules |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:8. |
| Doubling Time |
Approximately 24 hours |
| Recommended Medium |
F12K + 10% FBS + 6 μg/ml Puromycin |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Stability |
Stable in culture over 10 passages |
| QC |
WB |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
