Human FIBCD1-FGL1 Stable CHOK1 Cell Line
| Catalog Number |
CBCL-555 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
CHOK1 cell line stable expressing exogenous human FIBCD1-FGL1 fusion gene
Application
Drug screening and biological assays
Background
Fibrinogen-Like protein 1 (FGL1) is a liver specific secreted protein which belongs to the fibrinogen superfamily. FGL1 plays an important role in the development of hepatocellular carcinoma, with its elevated level in plasma of cancer patient indicating poor prognoses. Further studies showed that FGL1 can inhibit antigen-specific T cell activation through binding with immune checkpoint inhibiting molecule LAG3 through its fibrinogen-like domain independent of MHCII molecules.
Product Information
| Species |
Mouse |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
F12K + 10% FBS + 6 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Epithelial |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:8. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 24 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
