Human GPC3 Stable 293T Cell Line
| Catalog Number |
CBCL-495 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
HEK293T cell line stable expressing exogenous human GPC3 gene
Application
Drug screening and biological assays
Background
GPC3 is a family of heparan sulfate proteoglycans, which plays a role in the control of cell division and growth regulation. GPC3 is also found to be overexpressed in differentiating hepatocellular carcinoma (HCC) and dysplastic changes in cirrhotic livers, which might have the utility for diagnosis or treatment in cancer patient.
Product Information
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
DMEM + 10% FBS + 0.5 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
