Human GPC3 Stable 293T Cell Line
| Catalog Number |
CBCL-495 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
HEK293T cell line stable expressing exogenous human GPC3 gene
Application
Drug screening and biological assays
Background
GPC3 is a family of heparan sulfate proteoglycans, which plays a role in the control of cell division and growth regulation. GPC3 is also found to be overexpressed in differentiating hepatocellular carcinoma (HCC) and dysplastic changes in cirrhotic livers, which might have the utility for diagnosis or treatment in cancer patient.
Product Information
| Category |
Immune Molecules Cell Lines |
| Cell Line Name |
Human GPC3 Stable 293T Cell Line |
| Pharmaceutical Target |
Immune Molecules |
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Monolayer |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Doubling Time |
Approximately 30 hours |
| Recommended Medium |
DMEM + 10% FBS + 0.5 μg/ml Puromycin |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Stability |
Stable in culture over 10 passages |
| QC |
WB |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
