Human GPRC5D Stable 293T Cell Line
| Catalog Number |
CBCL-304 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable HEK293T cell line expressing exogenous human GPRC5D gene
Application
Drug screening and biological assays
Background
GPRC5D is a membrane of the G protein-coupled receptor family, and is predominately expressed on malignant bone marrow plasma cells, whereas its normal tissue expression is limited. Several therapeutical antibodies, and CART against GPCRSD have been developed rapidly to treat the multiple myeloma.
Product Information
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
DMEM + 10% FBS + 1 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
