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Human LAG3 Stable CHOK1 Cell Line

Catalog Number CBCL-578
Price Online Inquiry
Unit Quantity 1 x 10^6 cells / 1.0 ml

Introduction

The CHO-K1-Lag3-Stable Cell Line consists of recombinant CHO-K1 cells that stably overexpress human lymphocyte activating 3 (LAG3) on their surface. The surface expression of LAG3 is confirmed by FACS analysis.

Application

Drug screening and biological assays

Background

LAG-3 (Lymphocyte-activation gene 3) is a transmembrane protein expressed on activated T cells, NK cells, and B cells. It binds to MHC class II molecules with high affinity, inhibiting T cell proliferation and cytokine production. LAG-3 synergizes with PD-1 to promote T cell exhaustion in chronic infections and cancer, making it a potential target for immunotherapy.

Product Information

Category Immune Checkpoint Cell Lines
Cell Line Name CHO-K1-Lag3-Stable Cell Line
Pharmaceutical Target Immune Molecules
Protein Family Lymphocyte activation gene-3 family
Gene Lag3
NCBI Gene ID 3902
Species Hamster
Host Cell Line CHOK1
Cell Type Chinese hamster ovary cell line
Morphology Epithelial
Growth Properties Adherent
Selection Marker Puromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:8.
Doubling Time Approximately 24 hours
Recommended Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin
Freeze Medium 95% complete growth medium + 5% DMSO
Storage Method Frozen in liquid nitrogen.
Stability Validated for at least 10 passages
QC WB
Mycoplasma Negative
Sterility Testing Negative
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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