Human LILRB2 Stable 293T Cell Line

Catalog Number	CBCL-600
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable HEK293T cell line expressing exogenous human LILRB2 gene

Application

Drug screening and biological assays

Background

Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) belongs to the leukocyte immunoglobulin-like receptor (LIR) family of transmembrane glycoproteins that negatively regulate immune cell activation.LILRB2 is expressed on both innate (monocytes, macrophages, basophils, and DCs) and adaptive (CD4+ T cells) immune cells and interacts with MHC-I on nucleated cells (unknown specific ligand). LILRB2 interacts with related ligands HLA-G, ANGPTLs, SEMA4A and CD1d in tumor microenvironment, resulting in myeloid cells promoting tumor growth and enhancing tumor immune escape.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human LILRB2 Stable 293T Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Morphology	Monolayer
Growth Properties	Adherent
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 30 hours
Recommended Medium	DMEM + 10% FBS + 1 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.