Human LILRB2 Stable MC38 Cell Line

Catalog Number	CBCL-613
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable MC38 clone expressing exogenous human LILRB2 gene

Application

Drug screening and biological assays

Background

Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2), also known as ILT4, LIR2, monocyte/ macrophage immunoglobulin-like receptor 10, MIR-10, and CD85d, belongs to the leukocyte immunoglobulin-like receptor (LIR) family of transmembrane glycoproteins that negatively regulate immune cell activation. LILRB2 is expressed on both innate (monocytes, macrophages, basophils, and DCs) and adaptive (CD4+ T cells) immune cells and interacts with MHC-I on nucleated cells. LILRB2 interacts with related ligands hLA-G, ANGPTLs, SEMA4A and CD1d in tumor microenvironment, resulting in myeloid cells promoting tumor growth and enhancing tumor immune escape.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human LILRB2 Stable MC38 Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	MC38
Cell Type	Mouse colon cancer cell line
Morphology	Epithelial
Growth Properties	Adherent
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:10.
Doubling Time	Approximately 20 hours
Recommended Medium	RPMI1640 + 10% FBS + 5 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.