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Human LILRB4 Stable Ba/F3 Cell Line

Catalog Number CBCL-315
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable Ba/F3 cell line expressing exogenous human LILRB4 gene

Application

Drug screening and biological assays

Background

Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K) or ILT3, which is a member of leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 is receptor for class I MHC antigens. LILRB4 involved in the down-regulation of the immune response and the development of tolerance and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Product Information

Species Mouse
Host Cell Line Ba/F3
Cell Type Pro B cells
Stability Stable in culture over 10 passages
Growth Properties Suspension
Pharmaceutical Target Immune Molecules
Recommended Medium RPMI-1640 + 10% FBS + 8 ng/mL mIL-3+ 1 μg/ml Puromycin
Selection Marker Puromycin
Morphology Mostly round
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 20 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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