Human MDR1 Stable MDCK Cell Line

Catalog Number	CBCL-334
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable MDCK cell line expressing exogenous human MDR1 gene

Application

Drug screening and biological assays

Background

MDR1, also known as ABCB1, is a glycoprotein which is a well-characterized ABC-transporter of the MDR/TAP subfamily. MDR1 is an important protein of the cell membrane that pumps many foreign substances out of cells, and itisan ATP-dependent efflux pump with broad substrate specificity. MDR1 is expressed primarily in certain cell types in the liver, pancreas, kidney, colon, and jejunum. MDR1 is also found in brain capillary endothelial cells.

Product Information

Species	Dog
Host Cell Line	MDCK
Cell Type	Dog canine kidney cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	DMEM + 10% FBS + 2 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Monolayer
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.