Human MSLN Stable CHOK1 Cell Line

Catalog Number	CBCL-532
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

CHOK1 cell line stable expressing exogenous human MSLN gene

Application

Drug screening and biological assays

Background

Mesothelin, also shorted as MSLN, is a membrane protein expressed in mesothelial cells, and its biological function is mainly involved in cell adhesion. MSLN is overexpressed in various type of human tumors, including mesothelia, ovarian and pancreatic cancer. The identification of tumor marker lead to development of antitumor therapeutics, such as bispecific antibody, ADC, and tumor vaccine.

Product Information

Species	Mouse
Host Cell Line	CHOK1
Cell Type	Chinese hamster ovary cell line
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	F12K + 10% FBS + 6 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:8.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 24 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.