Human NKG2A Stable 293T Cell Line
| Catalog Number |
CBCL-400 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable HEK293T cell line expressing exogenous human NKG2A gene
Application
Drug screening and biological assays
Background
NKG2 is a type II transmembrane protein predominately expressed in NK cells. NKG2 has two isoforms, NKG2A and NKG2B, NKG2A can form heterodimers with CD94, linked by disulfide bonds and play an inhibitory role in immunological process after recognizes non-classical major histocompatibility (MHC) class Ib molecule HLA-E loaded with self-peptides derived from the signal sequence of classical MHC diss la molecules.
Product Information
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
DMEM + 10% FBS + 0.5 μg/ml Puromycin |
| Selection Marker |
Puromycin and hygromycin B |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
