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Human OX40L Stable 293T Cell Line

Catalog Number CBCL-474
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human OX40 Ligand gene

Application

Drug screening and biological assays

Background

OX40L, also named CD252 and TNSF4, is a type II transmembrane glycoprotein belonging to TNF superfamily, OX40L is the ligand for OX40 (CD134) and mainly expressed on the surface of activated B cells, T cells, DCs and endothelial cells. The interaction of OX40 and OX40L play a important role in late co-stimulatory signal to enhance the survival and proliferation of activated T cells.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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