Human OX40L Stable 293T Cell Line

Catalog Number	CBCL-474
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human OX40 Ligand gene

Application

Drug screening and biological assays

Background

OX40L, also named CD252 and TNSF4, is a type II transmembrane glycoprotein belonging to TNF superfamily, OX40L is the ligand for OX40 (CD134) and mainly expressed on the surface of activated B cells, T cells, DCs and endothelial cells. The interaction of OX40 and OX40L play a important role in late co-stimulatory signal to enhance the survival and proliferation of activated T cells.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human OX40L Stable 293T Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Morphology	Monolayer
Growth Properties	Adherent
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 30 hours
Recommended Medium	DMEM + 10% FBS + 0.5 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.