Human PD-L1 Stable CHOK1 Cell Line
| Catalog Number |
CBCL-415 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
The CHO-K1-PD-L1-Stable Cell Line consists of recombinant CHO-K1 cells that stably overexpress human programmed cell death 1 ligand 1 (PD-L1) on their surface. The surface expression of PD-L1 is confirmed by FACS analysis. This cell line is recommended for cell-based binding assays to screen antibodies against PD-L1 or to measure the binding affinity between PD-L1 and anti-PD-L1 antibodies.
Application
Drug screening and biological assays
Background
PDL1 (Programmed Death-Ligand 1) is an immune checkpoint protein that is expressed on the surface of tumor cells and some immune cells. It binds to its receptor PD-1 on T-cells, inhibiting T-cell activation and promoting immune tolerance, which helps tumors evade immune surveillance.
Product Information
| Category |
Immune Checkpoint Cell Lines |
| Cell Line Name |
CHO-K1-PD-L1-Stable Cell Line |
| Pharmaceutical Target |
Immune Molecules |
| Protein Family |
Programmed cell death ligand 1 family |
| Gene |
PD-L1 |
| NCBI Gene ID |
29126 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:8. |
| Doubling Time |
Approximately 24 hours |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin |
| Freeze Medium |
95% complete growth medium + 5% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| QC |
WB |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
