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Human PD-L1 Stable CHOK1 Cell Line

Catalog Number CBCL-415
Price Online Inquiry
Unit Quantity 1 x 10^6 cells / 1.0 ml

Introduction

The CHO-K1-PD-L1-Stable Cell Line consists of recombinant CHO-K1 cells that stably overexpress human programmed cell death 1 ligand 1 (PD-L1) on their surface. The surface expression of PD-L1 is confirmed by FACS analysis. This cell line is recommended for cell-based binding assays to screen antibodies against PD-L1 or to measure the binding affinity between PD-L1 and anti-PD-L1 antibodies.

Application

Drug screening and biological assays

Background

PDL1 (Programmed Death-Ligand 1) is an immune checkpoint protein that is expressed on the surface of tumor cells and some immune cells. It binds to its receptor PD-1 on T-cells, inhibiting T-cell activation and promoting immune tolerance, which helps tumors evade immune surveillance.

Product Information

Category Immune Checkpoint Cell Lines
Cell Line Name CHO-K1-PD-L1-Stable Cell Line
Pharmaceutical Target Immune Molecules
Protein Family Programmed cell death ligand 1 family
Gene PD-L1
NCBI Gene ID 29126
Species Hamster
Host Cell Line CHOK1
Cell Type Chinese hamster ovary cell line
Morphology Epithelial
Growth Properties Adherent
Selection Marker Puromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:8.
Doubling Time Approximately 24 hours
Recommended Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin
Freeze Medium 95% complete growth medium + 5% DMSO
Storage Method Frozen in liquid nitrogen.
Stability Validated for at least 10 passages
QC WB
Mycoplasma Negative
Sterility Testing Negative
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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