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Human PD-L1 Stable MiaPaca2 Cell Line

Catalog Number CBCL-416
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

MiaPaca2 cell line stable expressing exogenous human PDL1 gene

Application

Drug screening and biological assays

Background

PD-L1, also called human programed cell death ligand 1, is a transmembrane protein that play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease, virus infection and cancer. PD-L1 binds to its receptor PD-1 on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition. Upregulation of PD-L1 can allow cancer cell to evade the host immune system.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name Human PD-L1 Stable MiaPaca2 Cell Line
Pharmaceutical Target Immune Molecules
Species Human
Host Cell Line MiaPaca2
Cell Type Human pancreatic cancer cell line
Morphology Monolayer
Growth Properties Adherent
Selection Marker Puromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:3-1:4.
Recommended Medium RPMI-1640 + 10% FBS + 1 μg/ml Puromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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