Human PD1 Stable H22 Cell Line
| Catalog Number |
CBCL-282 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
The H22-PD1-Stable Cell Line is a stable clone of the H22 cell line that expresses the exogenous PD1 gene.
Application
Drug screening and biological assays
Background
PD-1 (Programmed Death-1) is an immune checkpoint receptor expressed on activated T-cells. It binds to its ligands PD-L1 and PD-L2, inhibiting T-cell activation and promoting immune tolerance, which is exploited by tumors to evade immune surveillance.
Product Information
| Category |
Immune Molecules Cell Lines |
| Cell Line Name |
H22-PD1-Stable Cell Line |
| Pharmaceutical Target |
Immune Molecules |
| Protein Family |
Programmed cell death protein 1 family |
| Gene |
PD1 |
| NCBI Gene ID |
5133 |
| Species |
Mouse |
| Host Cell Line |
H22 |
| Cell Type |
Mouse hepatoma |
| Morphology |
Fibroblast-like |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:10. |
| Doubling Time |
Approximately 20 hours |
| Recommended Medium |
RPMI1640+10%FBS+1µg/mL Puromycin |
| Freeze Medium |
70% RPMI1640+20% FBS+10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| QC |
WB |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
