Human PD11 Stable M-07e Cell Line

Catalog Number	CBCL-316
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable M-07e cell line expressing exogenous human PD1 gene

Application

Drug screening and biological assays

Background

PD-1, also named CD279, is a receptor belonging to immunoglobulin superfamily which is expressed primarily on activated T cells. NK cells, B cells, and monocytes. PD-1 interaction with its ligands, PD-L1 and PD-L2, leads to downregulation of T cell responses, including T cell proliferation and cytokine production, and limits immune-destruction of tissues. The interaction between PD-1 and its ligands, thus plays a role in maintaining the balance between immune activation and tolerance, potentially including tumor tolerance.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human PD11 Stable M-07e Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	M-07e
Cell Type	Acute megakaryoblast leukemia cell line
Morphology	Mostly round
Growth Properties	Suspension
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 40 hours
Recommended Medium	RPMI1640 + 10% FBS + GM-CSF (10 ng/mL) + 1 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.