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Human PDL1 Stable HepG2 Cell Line

Catalog Number CBCL-406
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable HepG2 clone expressing exogenous human PDL1 gene

Application

Drug screening and biological assays

Background

PD-L1, also called human programmed cell death ligand 1, is a transmembrane protein that plays a significant role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease, virus infection, and cancer. PD-L1 binds to its receptor PD-1 on activated T cells, B cells, and myeloid cells to modulate activation or inhibition. Upregulation of PD-L1 can allow the cancer cell to evade the host immune system.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name Human PDL1 Stable HepG2 Cell Line
Pharmaceutical Target Immune Molecules
Species Human
Host Cell Line HepG2
Cell Type Human Liver Cancer Cell Line
Morphology Epithelial
Growth Properties Adherent
Selection Marker Puromycin and hygromycin B
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Doubling Time Approximately 40 hours
Recommended Medium RPMI1640 + 10% FBS+ 1 μg/ml Puromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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