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Human PDL1 Stable Raji Cell Line

Catalog Number CBCL-418
Price Online Inquiry
Unit Quantity 5 x 10^6 cells / 1.0 ml

Introduction

The Raji-Human PD-L1-Stable Cell Line is engineered to express full-length human PD-L1 (Uniprot: Q9NZQ7-1), used to mimic cancer target cells. When co-cultured with human PD-1 Reporter Cell, the PD-1/PD-L1 interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the PD-1/PD-L1 interaction by either anti-PD-1 or anti-PD-L1 antibodies releases the inhibitory signal and results in TCR activation and NFAT-mediated luminescence.

Application

Useful for cell-based PD-L1 binding assay

Background

PDL1 (Programmed Death-Ligand 1) is an immune checkpoint protein that is expressed on the surface of tumor cells and some immune cells. It binds to its receptor PD-2 on T-cells, inhibiting T-cell activation and promoting immune tolerance, which helps tumors evade immune surveillance.

Product Information

Category Immune Checkpoint Cell Lines
Cell Line Name Raji-Human PD-L1-Stable Cell Line
Pharmaceutical Target Immune Molecules
Protein Family Programmed cell death ligand 1 family
Gene PD-L1
NCBI Gene ID 29126
Species Human
Host Cell Line Raji
Cell Type Human B-cell lymphoma
Morphology Lymphoblastoid
Growth Properties Suspension
Selection Marker Puromycin (2 μg/mL)
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:8.
Doubling Time Approximately 24-36 hours
Recommended Medium RPMI-1640 + 10% FBS
Freeze Medium Serum-free cell cryopreservation medium
Storage Method Frozen in liquid nitrogen.
Stability Validated for at least 10 passages
QC WB
Mycoplasma Negative
Sterility Testing Negative
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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