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Human Siglec10 Stable 293T Cell Line

Catalog Number CBCL-608
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable HEK293T cell line expressing exogenous human siglec10 gene

Application

Drug screening and biological assays

Background

Siglec10, also named sialic acid binding Ig like lectin 10, is a member of siglecs, immunoglobulin super family lectins with a defining feature of recognizing sialic acid-containing structures. Since most of the siglec family members contain ITIM-or ITIM-like motifs and many are known to be associated with SHP-1 and/or SHP-214, it has been suggested that the siglec family member may serve as a negative regulatory pathway for cellular activation.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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