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Human Siglec15 Stable 293T Cell Line

Catalog Number CBCL-573
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human Siglec15 gene

Application

Drug screening and biological assays

Background

Siglec-15 is a transmembrane glycoprotein, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding |g-like lectin) family, and is found primarily on the surface of immune cells. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Epithelial
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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