Human Siglec15 Stable THP-1 Cell Line
| Catalog Number |
CBCL-615 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
THP-1 cell line stable expressing exogenous human siglec15 gene
Application
Drug screening and biological assays
Background
Siglec-15 is a transmembrane glycoprotein, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding lg-like lectin) family, and is found primarily on the surface of immune cells. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Product Information
| Species |
Human |
| Host Cell Line |
THP-1 |
| Cell Type |
Monocytes |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Suspension |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
RPMI1640 + 10% FBS + 1 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Mostly round |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 48 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
