Human SIRPa1 Stable CHOK1 Cell Line

Catalog Number	CBCL-636
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

CHOK1 cell line stable expressing exogenous human SIRPa1 gene

Application

Drug screening and biological assays

Background

Signal regulatory protein alpha (SIPR alpha), also named CD172a, is a regulatory membrane glycoprotein. SIRPa is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells, and also stem cells or neurons. SIRPa acts as inhibitory receptor through recognizing with a broadly expressed transmembrane protein CD47, this interaction also calls “don’t eat me” signal, and negatively regulates phagocytosis of innate immune cells.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human SIRPa1 Stable CHOK1 Cell Line
Pharmaceutical Target	Immune Molecules
Species	Hamster
Host Cell Line	CHOK1
Cell Type	Chinese hamster ovary cell line
Morphology	Monolayer
Growth Properties	Adherent
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:8.
Doubling Time	Approximately 24 hours
Recommended Medium	F12K + 10% FBS + 6 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.