Human TIGIT Stable CHOK1 Cell Line

Catalog Number	CBCL-397
Price	Online Inquiry
Unit Quantity	1 x 10^6 cells / 1.0 ml

Introduction

The CHO-K1-TIGIT-Stable Cell Line consists of recombinant CHO-K1 cells that stably overexpress human T cell immunoreceptor with Ig and ITIM domains (TIGIT) on their surface.

Application

Drug screening and biological assays

Background

TIGIT (T cell immunoreceptor with Ig and ITIM domains) is an inhibitory receptor expressed on T cells and NK cells. It competes with CD226 for binding to CD155/PVR on target cells, delivering an inhibitory signal that suppresses immune cell activation. TIGIT is upregulated in tumor-infiltrating lymphocytes and contributes to immune evasion in cancer.

Product Information

Category	Immune Checkpoint Cell Lines
Cell Line Name	CHO-K1-TIGIT-Stable Cell Line
Pharmaceutical Target	Immune Molecules
Protein Family	T cell immunoreceptor with Ig and ITIM domains family
Gene	TIGIT
Species	Hamster
Host Cell Line	CHOK1
Cell Type	Chinese hamster ovary cell line
Morphology	Epithelial
Growth Properties	Adherent
Selection Marker	Puromycin and hygromycin B
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:8.
Doubling Time	Approximately 24 hours
Recommended Medium	F12K, 10% FBS, 6 μg/ml Puromycin
Freeze Medium	95% complete growth medium + 5% DMSO
Storage Method	Frozen in liquid nitrogen.
Stability	Validated for at least 10 passages
QC	WB
Mycoplasma	Negative
Sterility Testing	Negative
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.