Catalog Number | CBCL-397 |
Price | Online Inquiry |
Unit Quantity | 1 x 10^6 cells / 1.0 ml |
The CHO-K1-TIGIT-Stable Cell Line consists of recombinant CHO-K1 cells that stably overexpress human T cell immunoreceptor with Ig and ITIM domains (TIGIT) on their surface.
Drug screening and biological assays
TIGIT (T cell immunoreceptor with Ig and ITIM domains) is an inhibitory receptor expressed on T cells and NK cells. It competes with CD226 for binding to CD155/PVR on target cells, delivering an inhibitory signal that suppresses immune cell activation. TIGIT is upregulated in tumor-infiltrating lymphocytes and contributes to immune evasion in cancer.
Category | Immune Checkpoint Cell Lines |
Cell Line Name | CHO-K1-TIGIT-Stable Cell Line |
Pharmaceutical Target | Immune Molecules |
Protein Family | T cell immunoreceptor with Ig and ITIM domains family |
Gene | TIGIT |
Species | Hamster |
Host Cell Line | CHOK1 |
Cell Type | Chinese hamster ovary cell line |
Morphology | Epithelial |
Growth Properties | Adherent |
Selection Marker | Puromycin and hygromycin B |
Seeding Density | Thaw entire contents into an appropriate T25 flask. |
Subculture Ratio | Recommended a ratio of 1:4-1:8. |
Doubling Time | Approximately 24 hours |
Recommended Medium | F12K, 10% FBS, 6 μg/ml Puromycin |
Freeze Medium | 95% complete growth medium + 5% DMSO |
Storage Method | Frozen in liquid nitrogen. |
Stability | Validated for at least 10 passages |
QC | WB |
Mycoplasma | Negative |
Sterility Testing | Negative |
Shipping | Shipping in dry ice |
Biosafety Level | BSL-1 |
Caution | For Research Use Only |