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Human TIM3 Stable THP-1 Cell Line

Catalog Number CBCL-515
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

THP-1 cell line stable expressing exogenous human TIM3 gene

Application

Drug screening and biological assays

Background

T cell immunoglobulin and mucin-domain containing-3 (TIM-3), also known as Hepatitis A virus cellular receptor 2 (HAVCR2), is a member of TIM family of immune relegating molecules. TIM3 is expressed on multiple activated immune cells including macrophage, monocytes, dendritic cells, Th1, CD8+ T cells and NK cells. Recent studies showed that TIM3 play animportant role as an additional immune checkpoint and a promising drug target for cancer therapy.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name Human TIM3 Stable THP-1 Cell Line
Pharmaceutical Target Immune Molecules
Species Human
Host Cell Line THP1
Cell Type Monocytes
Morphology Mostly round
Growth Properties Suspension
Selection Marker Puromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Doubling Time Approximately 48 hours
Recommended Medium RPMI1640 + 10% FBS + 500 μg/ml Puromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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