Human TIM3 Stable THP-1 Cell Line

Catalog Number	CBCL-515
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

THP-1 cell line stable expressing exogenous human TIM3 gene

Application

Drug screening and biological assays

Background

T cell immunoglobulin and mucin-domain containing-3 (TIM-3), also known as Hepatitis A virus cellular receptor 2 (HAVCR2), is a member of TIM family of immune relegating molecules. TIM3 is expressed on multiple activated immune cells including macrophage, monocytes, dendritic cells, Th1, CD8+ T cells and NK cells. Recent studies showed that TIM3 play animportant role as an additional immune checkpoint and a promising drug target for cancer therapy.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human TIM3 Stable THP-1 Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	THP1
Cell Type	Monocytes
Morphology	Mostly round
Growth Properties	Suspension
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 48 hours
Recommended Medium	RPMI1640 + 10% FBS + 500 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.