Human TPBG Stable B16F10 Cell Line

Catalog Number	CBCL-290
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable B16F10 cell line expressing exogenous human TPBG gene

Application

Drug screening and biological assays

Background

TPBG, also known as 574, is a type I transmembrane glycoprote in that may be involved in cell adhesion, which is an antagonist of Wnt/B-catenin signalling pathway. TPBG is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. Its confined expression appears to give TPBG the potential to be a target for T cells in cancer immunotherapy.

Product Information

Species	Mouse
Host Cell Line	B16F10
Cell Type	Mouse skin melanoma cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	DMEM + 10% FBS + 1 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Mixture of spindle-shaped and epithelial-like cells
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 30 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.