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Human type II IL4R Stable 293T Cell Line

Catalog Number CBCL-638
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable HEK293T cell line expressing exogenous human IL4Ra & IL13R1 gene

Application

Drug screening and biological assays

Background

IL4 receptor complex is composed of IL-4Ra subunit and one accessory protein, the common cytokine receptor chain or IL-13Ra1, both of which transduce signals through IL4Ra. Type I IL-4R complexes, composed of IL-4Ra and common cytokine receptor, dominate in hematopoietic lineages and exclusively bind to IL-4 but not to IL-13.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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