IGF1R Stable Ba/F3 Cell Line
| Catalog Number |
CBCL-148 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable Ba/F3 clone expressing exogenous IGF1R sequence.
Application
Drug screening and biological assays
Background
IGF1R also named CD221, is a receptor tryrosin kinase activated by a hormone IGF1 and a related hormone IGF2. IGF1R plays an important role in growth, anabolism and body size. The overactivation of IGF1R due to increased level or mutation was found in several primary and metastatic cancer.
Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Product Information
| Species |
Mouse |
| Host Cell Line |
Ba/F3 cell line |
| Cell Type |
Pro-B cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Suspension |
| Pharmaceutical Target |
Kinases |
| Recommended Medium |
RPMI 1640 + 10% FBS + 100ng/ml IGF1 |
| Selection Marker |
Puromycin |
| Morphology |
Mostly round |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:10. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 20 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
