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JAK2-V617F Stable Ba/F3 Cell Line

Catalog Number CBCL-238
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable Ba/F3 clone expressing exogenous JAK2 bearing V617F amino acid mutation.

Application

Drug screening and biological assays

Background

Janus kinase 2 (commonly called JAK2) is a non-receptor tyrosine kinase. Mutations in JAK2 have been implicated in polycythemia vera, essential thrombocythemia, and myelofibrosis as well as other myeloproliferative disorders. Jak2 mutation, when demonstrable, is one of the methods of diagnosing polycythemia vera. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.

Product Information

Species Mouse
Host Cell Line Ba/F3 cell line
Cell Type Pro-B cells
Stability Stable in culture over 10 passages
Growth Properties Suspension
Pharmaceutical Target Kinases
Recommended Medium RPMI 1640 + 10% FBS
Selection Marker Puromycin
Morphology Mostly round
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 20 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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