JAK2-V617F Stable Ba/F3 Cell Line
| Catalog Number | CBCL-238 |
| Price | Online Inquiry |
| Unit Quantity | 1x106 cells / 1.0 ml |
Introduction
Stable Ba/F3 clone expressing exogenous JAK2 bearing V617F amino acid mutation.
Application
Drug screening and biological assays
Background
Janus kinase 2 (commonly called JAK2) is a non-receptor tyrosine kinase. Mutations in JAK2 have been implicated in polycythemia vera, essential thrombocythemia, and myelofibrosis as well as other myeloproliferative disorders. Jak2 mutation, when demonstrable, is one of the methods of diagnosing polycythemia vera. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Product Information
| Category | Kinase Cell Lines |
| Cell Line Name | JAK2-V617F Stable Ba/F3 Cell Line |
| Pharmaceutical Target | Kinases |
| Species | Mouse |
| Host Cell Line | Ba/F3 |
| Cell Type | Pro-B cells |
| Morphology | Mostly round |
| Growth Properties | Suspension |
| Selection Marker | Puromycin |
| Seeding Density | Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio | Recommended a ratio of 1:10. |
| Doubling Time | Approximately 20 hours |
| Recommended Medium | RPMI 1640 + 10% FBS |
| Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Stability | Stable in culture over 10 passages |
| QC | WB |
| Shipping | Shipping in dry ice |
| Biosafety Level | BSL-1 |
| Caution | For Research Use Only |
For research use only. Not for any other purpose.