Jurkat-Human CD16a (158V) (Luc)-Reporter Cell
| Catalog Number |
CBCL-0241 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This cell line is a Jurkat-based reporter cell line engineered to express the NFAT response element-driven luciferase expression system and the full-length human CD16a (Uniprot: P08637) with a Valine (V) mutation at amino acid 158. Compared to CD16a-158F, it has a higher affinity for IgG1 and IgG3 isotypes. It is used to evaluate the ADCC activity of antibodies in the presence of corresponding target cells.
Application
Determination of ADCC activity induced by antibodies
Background
CD16a is a low-affinity Fc gamma receptor with polymorphisms affecting its affinity for IgG. The 158V variant has higher affinity for IgG.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
Jurkat-Human CD16a (158V) (Luc)-Reporter Cell |
| Protein Family |
Fc gamma receptor family |
| Gene |
CD16a (158V) |
| NCBI Gene ID |
2215 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
