Jurkat-Human CD32a (131H) (Luc)-Reporter Cell
| Catalog Number |
CBCL-0238 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human CD32a (131H) (Luc) Jurkat Reporter Cell was designed to express the NFAT response element driving luciferase expression and the full - length human CD32a (131H) (Uniprot: P12318 - 1), which has a higher affinity for IgG2 isotypes compared to CD32a - 131R. This cell line can be used to evaluate the ADCP activity of antibodies in the presence of corresponding target cells. When co - cultured with a target cell and a relevant antibody, the antibody binds to both the target cell antigen and the CD32a (131H) receptor on the surface of the Human CD32a (131H) Jurkat Reporter Cell, resulting in receptor clustering, intracellular signaling, and NFAT - mediated luminescence.
Application
Determination of ADCP activity induced by antibodies.
Background
CD32a is a low-affinity Fc gamma receptor with polymorphisms affecting its function. The 131H variant is associated with altered immune responses.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
Jurkat-Human CD32a (131H) (Luc)-Reporter Cell |
| Protein Family |
Fc gamma receptor family |
| Gene |
CD32a (131H) |
| NCBI Gene ID |
2213 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
