Jurkat-Human CD32a (131R) (Luc)-Stable Cell Line
| Catalog Number |
CBCL-0012 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
A Jurkat reporter cell line that expresses the NFAT response element driving luciferase - expressing systems and the full - length human CD32a (131R) (Uniprot: P12318-1) has been engineered. This cell line can be used to evaluate the ADCP activity of antibodies in the presence of corresponding target cells.
Application
Determination of ADCP activity induced by antibodies.
Background
FCGR2A encodes a receptor for the Fc region of IgG. It is widely expressed on various immune cells, including neutrophils, monocytes, macrophages, and platelets. This receptor is involved in phagocytosis, the process by which immune cells engulf and destroy pathogens or foreign particles. It also plays a role in the clearance of immune complexes and the regulation of inflammatory responses. Polymorphisms in this gene have been associated with various autoimmune and infectious diseases.
Product Information
| Category |
Fc Receptor Cell Lines |
| Cell Line Name |
Jurkat-Human CD32a (131R) (Luc)-Stable Cell Line |
| Protein Family |
Fc gamma receptor |
| Gene |
CD32a/FCGR2A |
| NCBI Gene ID |
2212 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
