Jurkat-Human CD32b (Luc)-Reporter Cell
| Catalog Number |
CBCL-0240 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This cell line is a Jurkat-based reporter cell line engineered to express the NFAT response element-driven luciferase expression system and the full-length human CD32b (Uniprot: P31994-1). It is used to evaluate the ADCP activity of antibodies in the presence of corresponding target cells.
Application
Determination of ADCP activity induced by antibodies.
Background
CD32b is a low-affinity Fc gamma receptor that inhibits immune cell activation and plays a role in maintaining immune tolerance.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
Jurkat-Human CD32b (Luc)-Reporter Cell |
| Protein Family |
Fc gamma receptor family |
| Gene |
CD32b |
| NCBI Gene ID |
2214 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
