Jurkat-Human CD32b (Luc)- Stable Cell Line
| Catalog Number |
CBCL-0016 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human CD32b (Luc) Jurkat Reporter Cell has been designed to express both the NFAT response element that drives luciferase expression and the full-length human CD32b receptor (Uniprot: P31994-1). This cell line can be utilized to assess the ADCP activity of antibodies in the presence of corresponding target cells.
Application
Determination of ADCP activity induced by antibodies.
Background
FCGR2B encodes an inhibitory receptor for the Fc region of IgG. It is expressed on B cells, macrophages, and dendritic cells. This receptor contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail. When the receptor is engaged by immune complexes, it recruits phosphatases that inhibit the activation signals generated by other immune receptors, thereby dampening the immune response. It plays a crucial role in maintaining immune tolerance and preventing autoimmune diseases.
Product Information
| Category |
Fc Receptor Cell Lines |
| Cell Line Name |
Jurkat-Human CD32b (Luc)- Stable Cell Line |
| Protein Family |
Fc gamma receptor |
| Gene |
CD32b/FCGR2B |
| NCBI Gene ID |
2213 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
