Jurkat-Human CD64 (Luc)-Reporter Cell
| Catalog Number |
CBCL-0237 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human CD64 (Luc) Jurkat Reporter Cell was designed to express the NFAT response element driving luciferase expression and the full - length human CD64 (Uniprot: P12314 - 1). This cell line can be used to evaluate the ADCP activity of antibodies in the presence of corresponding target cells. When co - cultured with a target cell and a relevant antibody, the antibody binds to both the target cell antigen and the CD64 receptor on the surface of the Human CD64 (Luc) Jurkat Reporter Cell, resulting in receptor clustering, intracellular signaling, and NFAT - mediated luminescence.
Application
Determination of ADCP activity induced by antibodies
Background
CD64 is a high-affinity Fc gamma receptor that binds IgG antibodies and plays a role in antibody-dependent cellular cytotoxicity and phagocytosis.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
Jurkat-Human CD64 (Luc)-Reporter Cell |
| Protein Family |
Fc gamma receptor family |
| Gene |
CD64 |
| NCBI Gene ID |
2209 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
