Jurkat-Human LAG-3 (Luc)-Reporter Cell

Introduction

This Jurkat reporter cell line expresses the NFAT response element driving luciferase expression systems and the full-length human LAG-3 (Uniprot: P18627-1) receptor, which can be used to evaluate the potency of LAG-3 blockade. When co-cultured with target cells expressing human MHCⅡ, the LAG-3/MHCⅡ interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the LAG-3/MHCⅡ interaction with anti-LAG-3 antibodies releases the inhibitory signals, resulting in TCR activation and NFAT-mediated luminescence.

Application

Screen for anti-human LAG-3 antibody.

Background

LAG-3 is an immune checkpoint receptor that inhibits T cell activation and proliferation, playing a role in immune tolerance and tumor immune evasion.

Product Information